I am supposed to synthesize tripeptide, Ala−Asn−Phe, using Merrifield peptide synthesis procedure. Accordingly, I have done followings:
- Attached protected Phe as the first amino acid.
- Got rid of protecting group (BOC)
- Added asparagine with 2 protecting groups - BOC for α-NHX2 group and FMOC for the γ-NHX2 group.
- Got rid of BOC and added Ala protected with BOC.
- Got rid of BOC and FMOC, and used HF to release the peptide.
Is this going to work? The thing that worries me the most is the γ-amino group and using FMOC - is that a good idea?
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