Thursday, November 14, 2019

biochemistry - Synthesis of tripeptide using Merrifield peptide synthesis procedure - Inclusion of asparagine


I am supposed to synthesize tripeptide, AlaAsnPhe, using Merrifield peptide synthesis procedure. Accordingly, I have done followings:




  1. Attached protected Phe as the first amino acid.

  2. Got rid of protecting group (BOC)

  3. Added asparagine with 2 protecting groups - BOC for α-NHX2 group and FMOC for the γ-NHX2 group.

  4. Got rid of BOC and added Ala protected with BOC.

  5. Got rid of BOC and FMOC, and used HF to release the peptide.


Is this going to work? The thing that worries me the most is the γ-amino group and using FMOC - is that a good idea?


enter image description here




No comments:

Post a Comment

digital communications - Understanding the Matched Filter

I have a question about matched filtering. Does the matched filter maximise the SNR at the moment of decision only? As far as I understand, ...