I am supposed to synthesize tripeptide, $\mathrm{Ala-Asn-Phe}$, using Merrifield peptide synthesis procedure. Accordingly, I have done followings:
- Attached protected $\mathrm{Phe}$ as the first amino acid.
- Got rid of protecting group ($\mathrm{BOC}$)
- Added asparagine with 2 protecting groups - $\mathrm{BOC}$ for $\alpha$-$\ce{NH2}$ group and $\mathrm{FMOC}$ for the $\gamma$-$\ce{NH2}$ group.
- Got rid of $\mathrm{BOC}$ and added $\mathrm{Ala}$ protected with $\mathrm{BOC}$.
- Got rid of $\mathrm{BOC}$ and $\mathrm{FMOC}$, and used $\ce{HF}$ to release the peptide.
Is this going to work? The thing that worries me the most is the $\gamma$-amino group and using $\mathrm{FMOC}$ - is that a good idea?
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